Sometimes when you click the Queue Prompt, nothing happens and it seems the “Queue Prompt” is not working.
One of the reasons is that there is a "caching" of ComfyUI, it will NOT queue the prompt if nothing changed in the workflow from the previous “Queue Prompt”.
Please see if you could find the control_after_generate in the KSampler and change it to randomized (means it will use a random seed number each time it generates anything), see the doc here https://blenderneko.github.io/ComfyUI-docs/Core%20Nodes/Sampling/KSampler%20Advanced/#ksampler-advanced.
Or just change the prompt a bit.